ネモト ナオキ   Naoki Nemoto
  根本 直樹
   所属   千葉工業大学  先進工学部 生命科学科
   千葉工業大学  工学研究科 工学専攻
   千葉工業大学  先進工学研究科 生命科学専攻
   職種   教授
言語種別 英語
発行・発表の年月 2023/11
形態種別 学術雑誌
査読 査読あり
標題 Profiling of lipids in Thermus thermophilus HB8 grown under various conditions.
執筆形態 共著
掲載誌名 The Journal of general and applied microbiology
掲載区分国外
巻・号・頁 69(2),pp.79-90
著者・共著者 Masahiko Kawaguchi, Haruo Shimada, Yoshitaka Bessho, Naoki Nemoto
概要 To clarify the whole lipid profile of Thermus thermophilus HB8, we cultured this organism under four different growth (temperature and/or nutrition) conditions and analyzed the compositions of polar lipids and fatty acids by high-performance thin-layer chromatography (HPTLC) and gas chromatograph-mass spectrometry (GCーMS), respectively. Thirty-one lipid spots were detected on HPTLC plates and profiled in terms of the presence or absence of phosphate, amino, and sugar groups. Then, we allocated ID numbers to all the spots. Comparative analyses of these polar lipids showed that the diversity of lipid molecules increased under high temperature and minimal medium conditions.
DOI 10.2323/jgam.2023.06.006
ISSN 1349-8037
PMID 37394432