カワイ　ゴウタ   Gota Kawai   河合　剛太    所属   千葉工業大学  先進工学部 生命科学科    千葉工業大学  工学研究科 工学専攻    千葉工業大学  先進工学研究科 生命科学専攻    職種   教授
言語種別	英語
発行・発表の年月	2018/12
形態種別	学術雑誌
査読	査読あり
標題	Polyamines stimulate the CHSY1 synthesis through the unfolding of the RNA G-quadruplex at the 50-untraslated region
執筆形態	共著
掲載誌名	Biochemical Journal
掲載区分	国外
出版社・発行元	Portland press
巻・号・頁	475(23),pp.3797-3812
著者・共著者	Katsutoshi Yamaguchi, Kiryu Asakura, Masataka Imamura, Gota Kawai, Taiichi Sakamoto, Tomomi Furihata, Robert J. Linhardt, Kazuei Igarashi, Toshihiko Toida and Kyohei Higashi
概要	We found that 4S/6S ratio and molecular weight of CS were decreased in polyamine-depleted cells. In addition, decreased levels of chondroitin synthase 1 (CHSY1) and chondroitin 4-O-sulfotransferase 2 proteins were also observed on polyamine depletion. Interestingly, the translation initiation of CHSY1 was suppressed by a highly structured sequence (positions −202 to −117 relative to the initiation codon) containing RNA G-quadruplex (G4) structures in 5'-untranslated region. The formation of the G4s was influenced by the neighboring sequences to the G4s and polyamine stimulation of CHSY1 synthesis disappeared when the formation of the G4s was inhibited by site-directed mutagenesis.