クマタニ　アキチカ   Akichika Kumatani   熊谷　明哉    所属   千葉工業大学  工学部 電気電子工学科    千葉工業大学  工学研究科 工学専攻    職種   教授
発行・発表の年月	2021/03/23
形態種別	学術雑誌
査読	査読あり
標題	Micropipet-Based Navigation in a Microvascular Model for Imaging Endothelial Cell Topography Using Scanning Ion Conductance Microscopy
執筆形態	共著
掲載誌名	Analytical Chemistry
掲載区分	国外
出版社・発行元	American Chemical Society (ACS)
巻・号・頁	93(11),4902-4908頁
著者・共著者	Noriko Taira,Yuji Nashimoto,Kosuke Ino,Hiroki Ida,Takuto Imaizumi,Akichika Kumatani,Yasufumi Takahashi,Hitoshi Shiku
概要	Scanning ion conductance microscopy (SICM) has enabled cell surface topography at a high resolution with low invasiveness. However, SICM has not been applied to the observation of cell surfaces in hydrogels, which can serve as scaffolds for three-dimensional cell culture. In this study, we applied SICM for imaging a cell surface in a microvascular lumen reconstructed in a hydrogel. To achieve this goal, we developed a micropipet navigation technique using ionic current to detect the position of a microvascular lumen. Combining this navigation technique with SICM, endothelial cells in a microvascular model and blebs were visualized successfully at the single-cell level. To the best of our knowledge, this is the first report on visualizing cell surfaces in hydrogels using a SICM. This technique will be useful for furthering our understanding of the mechanism of intravascular diseases.
DOI	10.1021/acs.analchem.0c05174
ISSN	0003-2700/1520-6882
PermalinkURL	https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c05174