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クマタニ アキチカ
Akichika Kumatani
熊谷 明哉 所属 千葉工業大学 工学部 電気電子工学科 千葉工業大学 工学研究科 工学専攻 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2017/12/13 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes |
| 執筆形態 | 共著 |
| 掲載誌名 | ACS Applied Materials and Interfaces |
| 掲載区分 | 国外 |
| 巻・号・頁 | 9(49),pp.42444-42458 |
| 国際共著 | 国際共著 |
| 著者・共著者 | Serge Ostrovidov,Majid Ebrahimi,Hojae Bae,Hung Kim Nguyen,Sahar Salehi,Sang Bok Kim,Akichika Kumatani,Tomokazu Matsue,Xuetao Shi,Ken Nakajima,Shizu Hidema,Makoto Osanai,Ali Khademhosseini |
| 概要 | In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation. |
| DOI | 10.1021/acsami.7b03979 |
| ISSN | 1944-8244/1944-8252 |
| PMID | 29023089 |