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ネモト ナオキ
Naoki Nemoto
根本 直樹 所属 千葉工業大学 先進工学部 生命科学科 千葉工業大学 工学研究科 工学専攻 千葉工業大学 先進工学研究科 生命科学専攻 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2024/10 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | Crystal structure of guanosine 5'-monophosphate synthetase from the thermophilic bacterium Thermus thermophilus HB8. |
| 執筆形態 | 共著 |
| 掲載誌名 | Acta crystallographica. Section F, Structural biology communications |
| 掲載区分 | 国外 |
| 出版社・発行元 | IUCr Journals |
| 巻・号・頁 | 80(Pt 10),pp.278-285 |
| 著者・共著者 | Naoki Nemoto, Seiki Baba, Gota Kawai, Gen Ichi Sampei |
| 概要 | In this study, the crystal structure of an XMP-bound form of Guanosine 5'-monophosphate (GMP) synthetase (GuaA) from the thermophilic bacterium Thermus thermophilus HB8 (TtGuaA) was determined at a resolution of 2.20 Å and that of an apo form of TtGuaA was determined at 2.10 Å resolution. TtGuaA forms a homodimer, and the monomer is composed of three domains, which is a typical structure for GuaA. Disordered regions in the crystal structure were obtained from the AlphaFold2-predicted model structure, and a model with substrates (Gln, XMP and ATP) was constructed for molecular-dynamics (MD) simulations. The structural fluctuations of the TtGuaA dimer as well as the interactions between the active-site residues were analyzed by MD simulations. |
| DOI | 10.1107/S2053230X2400877X |
| ISSN | 2053-230X |
| PMID | 39291305 |