Faculty Information - Sakamoto Taiichi

■ Office

Tsudanuma Campus, No.1 Building,5th floor room 020510

■ Academic background

1.	～1997/03	〔Doctorial Course〕, Graduate School, Division of Engineering, Yokohama National University, Accomplished credits for doctoral program
2.	1997/09/30 Degree Acquisition	Yokohama National University,

■ Research Overview

RNA molecules have been payed attention for their extremely important role in life. RNA is also used as medicine, and is expected to become effective therapeutics for diseases that have been difficult to treat until now. Furthermore, RNA research has begun on the application of RNA as a functional material. We hope to design functional RNA easily and use it in various fields such as the environment and medicine.

■ Present specialized field

Structural biochemistry, Biochemistry (Key Word：RNA Engineering)

■ E-Mail Address

■ Research activities

1.

2014/02～

International Society on Aptamers

■ Works

1.	Book	Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome Using switchSENSE Methods in Molecular Biology 2263,pp.341-350 (Collaboration) 2021/04/21
2.	Book	Kinetic and Thermodynamic Analyses of RNA–Protein Interactions Methods in Molecular Biology RNA Chaperones 2106,pp.119-128 (Collaboration) 2020/01
3.	Book	ITC Measurement for High-Affinity Aptamers Binding to Their Target Proteins Methods in Molecular Biology Microcalorimetry of Biological Molecules 1964,pp.119-128 (Collaboration) 2019/04
4.	Book	NMR studies on RNA Experimental approaches of NMR spectroscopy-Methodology and application to life science and material (Collaboration) 2017/12
5.	Book	Translational Control by cis RNA Elements, Bacteria Encyclopedia of Systems Biology (Single) 2013/07
6.	Article	Stereocontrolled Synthesis and NMR Analysis of PB/PO Chimeric Oligonucleotides Royal Society Open Science (Collaboration) 2025/02
7.	Article	Selection and characterization of aptamers targeting the Vif-CBFβ-ELOB-ELOC-CUL5 complex Journal of Biochemistry 176,pp.205-215 (Collaboration) 2024/05
8.	Article	Development of the 12-Base Short Dimeric Myogenetic Oligodeoxynucleotide That Induces Myogenic Differentiation BioTech 13,pp.11 (Collaboration) 2024/04/25
9.	Article	Homo-trimeric structure of the ribonuclease for rRNA processing, FAU-1, from Pyrococcus furiosus Journal of Biochemistry (Collaboration) 2024/02/01
10.	Article	Selection of aptamers using β-1,3-glucan recognition protein tagged proteins and curdlan beads Journal of Biochemistry 174,pp.433-440 (Collaboration) 2023/07/27
11.	Article	Synthesis of P‑Modified DNA from Boranophosphate DNA as a Precursor via Acyl Phosphite Intermediates 88(15),pp.10617-10631 (Collaboration) 2023/07/18
12.	Article	Structural basis for the interaction between the first SURP domain of the SF3A1 subunit in U2 snRNP and the human splicing factor SF1 Protein Science 31(10),pp.e4437 (Collaboration) 2022/09/27
13.	Article	Amelioration of Alzheimer’s disease by gut–pancreas–liver–3 brain interaction in an App knock-in mouse model Life 12(1),pp.34 (Collaboration) 2021/12/27
14.	Article	The N93D mutation of the human T-cell leukemia virus type -1 envelope glycoprotein found in symptomatic patients enhances neuropilin-1 b1 domain binding Biochim. Biophys. Acta 1869,pp.140708 (Collaboration) 2021/07/31
15.	Article	Specific Inhibition of FGF5-induced Cell Proliferation by RNA Aptamers Scientific Reports 11,pp.2976 (Collaboration) 2021/02
16.	Article	A G-quadruplex-forming RNA aptamer binds to the MTG8 TAFH domain and dissociates the leukemic AML1-MTG8 fusion protein from DNA FEBS Lettters 594,pp.3477-3489 (Collaboration) 2020/09
17.	Article	Altered‐specificity mutants of the HIV Rev arginine‐rich motif‐RRE IIB interaction (Collaboration) 2020/01
18.	Article	Binding interaction analysis of RNA aptamer-Fc region of human Immunoglobulin G using fragment molecular orbital calculation Chem. Phys. Lett. (Collaboration) 2020/01
19.	Article	Artificial cationic peptides that increase nuclease resistance of siRNA without disturbing RNAi activity Nucleosides Nucleotides and Nucleic Acids 38,pp.338-348 (Collaboration) 2019/05
20.	Article	Crystal structure and calcium-induced conformational changes of diacylglycerol kinase α EF-hand domains. Protein Sci. (Collaboration) 2019/01
21.	Article	Polyamines stimulate the CHSY1 synthesis through the unfolding of the RNA G-quadruplex at the 5'-untraslated region. Biochem. J. 475,pp.3797-3812 (Collaboration) 2018/12
22.	Article	Synthesis of 4'-C-aminoalkyl-2'-O-methyl modified RNA and their biological properties Bioorg. Med. Chem. 26,pp.3521-3534 (Collaboration) 2018/07
23.	Article	Thermodynamic study of aptamers binding to their target proteins Biochimie 145,pp.91-97 (Collaboration) 2018/02
24.	Article	Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses FEBS Open Bio 8,pp.264-270 (Collaboration) 2018/01
25.	Article	Conjugation of two RNA aptamers improves binding affinity to AML1 Runt domain 162,pp.432-436 (Collaboration) 2017/12
26.	Article	NMR study of aptamers Aptamers 1,pp.13-18 (Single) 2017/11
27.	Article	NMR monitoring of the SELEX process to confirm enrichment of structured RNA Sci. Reports 7,pp.283 (Collaboration) 2017/03
28.	Article	Selection, characterization and application of artificial DNA aptamer containing appended bases with sub-nanomolar affinity for a salivary biomarker Sci. Reports 7,pp.42716 (Collaboration) 2017/03
29.	Article	Solution structure of the first RNA recognition motif domain of human spliceosomal protein SF3b49 and its mode of interaction with a SF3b145 fragment Protein Sci. 26,pp.280-291 (Collaboration) 2017/02
30.	Article	Two stems with different characteristics and an internal loop in an RNA aptamer contribute to spermine-binding J. Biochem. 161,pp.197-206 (Collaboration) 2017/02
31.	Article	Kinetic and thermodynamic analyses of interaction between a high-affinity RNA aptamer and its target protein Biochemistry 55,pp.6221-6229 (Collaboration) 2016/11
32.	Article	Altering the orientation of a fused protein to the RNA-binding ribosomal protein L7Ae and its derivatives through circular permutation Biochem. Biophys. Res. Commun. 466,pp.388-392 (Collaboration) 2015/10
33.	Article	A trifunctional, triangular RNA-protein complex FEBS Lett. 589,pp.2424-2428 (Collaboration) 2015/08
34.	Article	Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and S250 phosphorylation in the conserved C-terminal regions Biosci. Rep. 35,pp.e00228 (Collaboration) 2015/06
35.	Article	Solution structure of a DNA mimicking motif of an RNA aptamer against transcription factor AML1 Runt domain. Journal of Biochemistry 154(6),pp.513-519 (Collaboration) 2013/12
36.	Article	The Runt domain of AML1 (RUNX1) binds a sequence-conserved RNA motif that mimics a DNA element RNA 19(7),pp.927-936 (Collaboration) 2013/07
37.	Article	Conformational plasticity of RNA for target recognition as revealed by the 2.15 A crystal structure of a human IgG-aptamer complex Nucleic Acids Res. 38(21),pp.7822-7829 (Collaboration) 2010/11
38.	Article	Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus RNA 15(3),pp.420-431 (Collaboration) 2009/03
39.	Article	Crystallization and preliminary X-ray diffraction studies of an RNA aptamer in complex with the human IgG Fc fragment Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64,pp.942-944 (Collaboration) 2008/10
40.	Article	Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G RNA 14,pp.1154-1163 (Collaboration) 2008/06
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■ Home Page

http://www.lifescience.it-chiba.ac.jp/sakamoto/index_eng.html

■ Committee and society

1.

2017/09～

Aptamers Editorial Board Member